Collagen II Mouse anti-Bovine, Chicken, Human, Mouse, Rat, Clone: 2B1.5, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA137493

Catalog No. 01-671-784

Description

Staining of formalin/parafin tissues requires disgestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Specifications

• Antigen: Collagen II
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P02459, P02460, P02458, P28481, P05539
• Gene Alias: alpha-1 type II collagen, cartilage collagen, chondrocalcin, collagen II, alpha-1 polypeptide, collagen alpha-1(II) chain
• Host Species: Mouse
• Isotype: IgG2a, kappa
• Quantity: 200 μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 1280, 12824, 25412, 395069, 407142

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: 2B1.5
• Conjugate: Unconjugated
• Gene: COL2A1
• Format: Liquid
• Gene Symbols: COL2A1
• Immunogen: A purified preparation of lathyritic type II collagen from embryonic chicken sternum
• Purification Method: Protein A
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Bovine, Chicken, Human, Mouse, Rat