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Complement C5a Mouse anti-Human, Clone: 2942, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA140161

Catalog No. 01-676-534


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Description

Description

MA1-40161 detects C5a/C5a des-Arg from human samples. MA1-40161 has been successfully used in Western blot, ELISA, immunohistochemistry (frozen), and functional assay applications. By Western blot, the antibody detects a band size of 14 kDa. The MA1-40161 immunogen is the Neo-epitope on C5a. Positive control: Eye tissue of patient with AMD; Negative control: Eye tissue of patient without AMD. Endotoxin <24 EU/mg. General comment: Please notice that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products.

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and hematopoiesis.
Specifications

Specifications

Complement C5a
Monoclonal
0.1 mg/mL
PBS with 0.1% BSA and no preservative
P01031
CPAMD4, FLJ17816, FLJ17822, MGC142298
Mouse
IgG2a
100 μg
4° C
Primary
727
ELISA, Functional Assay, Immunohistochemistry (Frozen), Western Blot
2942
Unconjugated
C5
Liquid
C5
Neo-epitope on C5a.
Protein G
RUO
Antibody
Monoclonal
Human
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