COPII Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1069A
PA1-069A detects coatomer-protein II (COPII) from rat, mouse and human cells. PA1-069A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract. The PA1-069A immunogen is a synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII. This sequence is completely conserved between human, mouse, and rat. PA1-069A immunizing peptide (Cat. No. PEP-127) is available for use in neutralization and control experiments.Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta ft.-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p.
|SEC13, SEC23A, SEC24A, Sec31a|
|Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|O95486, Q5XFW8, Q3UPL0, Q9D1M0, P55735, Q15436, Q3U2P1, Q9Z2Q1, O94979, Q01405|
|-20° C, Avoid Freeze/Thaw Cycles|
|110379, 22872, 10802, 287275, 297522, 10484, 58817, 20334, 69162, 93646, 6396, 77371|
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