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Cyclophilin A Rabbit anti-Hamster, Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1025

Catalog No. PA1025


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Description

Description

PA1-025 detects recombit human cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. PA1-025 also detects the CyPA protein from CHO cells. PA1-025 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a prominent ~18 kDa protein representing recombit human CyPA. The PA1-025 immunogen is recombit human CyPA expressed in E. coli.

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase ft.s, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

Specifications

Cyclophilin A
Polyclonal
PBS with 0.05% sodium azide
P62937, P17742
CyP A, Peptidyl-prolyl cis-trans isomerase A, EC 5.2.1.8, PPIase, Rotamase, Cyclosporin A-binding protein
Rabbit
IgG
400 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
100769490, 268373, 5478
Immunocytochemistry, Immunofluorescence, Western Blot
Unconjugated
PPIA
Liquid
PPIA
Purified recombit human CyPA.
Ammonium sulfate precipitation
RUO
Antibody
Polyclonal
Hamster, Human, Mouse
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