D4-GDI Mouse anti-Human, Mouse, Clone: 97A1015, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA141085
Suggested positive control: anti-Fas treated Jurkat (10 ng/mL), antigen standard for ARHGDIB (transient overexpression lysate).D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
|PBS with 0.05% BSA and 0.05% sodium azide|
|Cleavage specific D4-GDI|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|Synthetic peptide corresponding to a region containing the Fas-induced cleavage site of human D4-GDI.|
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