Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

anti-DAAM2, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody

$119.60 - $221.00

Specifications

Host Species Rabbit
Isotype IgG
Immunogen A portion of amino acids 100-150 of human DAAM2 was used as the immunogen for this antibody.
Antigen DAAM2
Monoclonal or Polyclonal Polyclonal
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
NBP224536SS
View Documents
Novus Biologicals
NBP224536SS
0.025 mg
Each for $119.60
Add to cart
 
NBP224536
View Documents
Novus Biologicals
NBP224536
0.1 mg
Each for $221.00
Add to cart
 
Description

Description

DAAM2 Polyclonal antibody specifically detects DAAM2 in Human, Mouse, Rhesus Macaque samples. It is validated for Western Blot.
Specifications

Specifications

Rabbit
A portion of amino acids 100-150 of human DAAM2 was used as the immunogen for this antibody.
Polyclonal
Primary
Western Blot
23500
0.5 mg/mL
IgG
DAAM2
Affinity Purified
RUO
The amino acid sequence used as immunogen for this antibody is 100% homologous in human and rhesus monkey, 94% homologous in horse, 88% homologous in dog, and 76% homologous in cow, mouse and rat. For IF, the GLI1 IMG-6553A antibody was used at 10 ug/ml (Hu, 2010). Protocol: Cells were washed in PBS and fixed in 4% PFA, pH 7.4 (20g PFA in 500 ml PBS) for 10 min. The fixed cells were permeabilized in 0.5% Triton X-100 (in PBS) for 5 min and blocking in 50 ul of 20% HINGS (heat inactivated normal goat serum diluted in PBS) for 1 hr. The GLI1 antibody was incubated at 37 degrees C for 1 hr or at 4 degrees C overnight. Following 3 X 5 min washes in PBS, an AlexaFluor488 or 594-conjugated goat anti-rabbit antibody was used as a second step (diluted in 20% HINGS at 1:1000 for 1 hr at room temperature i the dark). Following 3 X 5 min washes in PBS, the slides were mounted with DABCO (1,4-diazabicyclo[2.2.2]octane) anti-fading agent for fluorescence microscopy.
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide
SDS
Product Certifications

For Research Use Only

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit