DAP5 Mouse anti-Human, Mouse, Clone: 39C534.1, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA141094
Suggested positive control: Jurkat, antigen standard for EIF4G2 (transient overexpression lysate), Jurkat whole cell lysate.Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4).
|PBS with 0.2% gelatin and 0.05% sodium azide|
|AAG1, DAP5, FLJ41344, NAT1, P97|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Flow Cytometry, Western Blot|
|Synthetic peptides corresponding to residues 796-812 of human DaP-5 protein.|
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