ICAD Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Supplier: Thermo Scientific PA133022
PA1-33022 detects DFF from human samples. PA1-33022 has been successfully used in immunoprecipitation, Western blot applications. The PA1-33022 immunogen is: Peptide: amino acids 2-21 at the N-terminus of human DFF Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.
|Antigen affinity chromatography
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
|Immunoprecipitation, Western Blot
|Peptide: amino acids 2-21 at the N-terminus of human DFF
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