ICAD Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA133021
PA1-33021 detects DFF from human samples. PA1-33021 has been successfully used in immunoprecipitation, Western blot applications. The PA1-33021 immunogen is: Peptide: amino acids 313-331 from the C-terminus of human DFF Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot|
|PBS with 0.02% sodium azide; pH 7.4|
|Peptide: amino acids 313-331 from the C-terminus of human DFF|
|Antigen affinity chromatography|
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