DNMT1 Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1880
PA1-880 detects DNA methyltransferase 1 (Dmnt1) from HeLa cell nuclear extracts. PA1-880 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~180 kDa protein representing Dnmt1 from HeLa cell nuclear extract. A doublet between 40 and 45 kDa is also seen on Western blots using this antibody. The PA1-880 immunogen is a synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1. PA1-880 immunizing peptide (Cat. No. PEP-136) is available for use in neutralization and control experiments.DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions. There exist three isoforms of Dnmts, Dnmt1, 2 and 3. Dnmt1 is believed to perform most of the maintece and de novo methylation activities that occur in somatic cells of mammals. Studies have shown that Dnmt1 can establish repression of transcription complexes consisting of Dnmt1, histone deacetylase 2 (HDAC2), and DNA methyltransferase 1 associated protein (DMAP1). Therefore, in addition to DNA methylation maintece, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication.
|Synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1.|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|-20° C, Avoid Freeze/Thaw Cycles|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok