POLI Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA529442
PA5-29442 targets DNA polymerase iota in WB applications and shows reactivity with Human samples. The PA5-29442 immunogen is recombit fragment corresponding to a region within amino acids 122 and 396 of Human DNA polymerase iota. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5 ft.-deoxyribose phosphate at abasic sites, but may not have lyase activity.
|Recombit fragment corresponding to a region within amino acids 122 and 396 of Human DNA polymerase iota|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7|
|RAD30B, RAD3OB, DNA polymerase iota, RAD30 homolog B, eta2, polymerase (DNA-directed), iota|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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