DYKDDDDK Tag Rabbit anti-Tag, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1984B
PA1-984B detects the DYKDDDDK epitope tag sequence, similar to the FLAG tag from Sigma. PA1-984B has been successfully used in Western blot and immunofluorescence procedures to detect the presence of fusion proteins containing the epitope tag sequence DYKDDDDK. PA1-984B immunizing peptide corresponds to the epitope tag sequence DYKDDDDKC. This peptide (Cat. No. PEP-087) is available for use in neutralization and control experiments.DYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombit DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
|-20° C, Avoid Freeze/Thaw Cycles|
|Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG Tag|
|Synthetic Peptide: D Y K D D D D K C|
|Antigen affinity chromatography|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok