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EBP50 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1090

Catalog No. PA1090


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Description

Description

PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells as well as recombit human protein. PA1-090 has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium. The PA1-090 immunogen is recombit human His-tagged EBP50 obtained from bacteria. All His-tag specific antibody has been removed. This product was epitope affinity purified using a synthetic peptide to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50.

EBP50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP50 is found exclusively at the apical membranes of epithelial cells.
Specifications

Specifications

EBP50
Polyclonal
Unconjugated
SLC9A3R1
Liquid
SLC9A3R1
Bacterially expressed, His-tagged human EBP50.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
O14745, P70441
Ezrin-Radixin-Moesin Binding Phosphoprotein 50, NHERF, Na(+)/H(+) exchange regulatory cofactor NHE-RF, Regulatory cofactor of Na(+)/H(+) exchanger, So
Rabbit
IgG
100 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
26941, 9368
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