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PCYT2 Rabbit anti-Human, Mouse, Non-human primate, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1545

Catalog No. PA1545

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PA1-545 detects ethanolaminephosphate cytidyltransferase (ECT) protein in human, rat, mouse and non-human primate samples. PA1-545 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing ECT from C3H/10T1/2 cell lysates. PA1-545 immunizing peptide corresponds to amino acid residues 157-172 from human ECT protein. This immunizing peptide (PEP-241) can be used for neutralization and control experiments.

ECT (ethanolaminephosphate cytidyltransferase) is an important regulatory enzyme in the pathway for phosphatidylethanolamine (PE) biosynthesis.  PE functions in cell signalling, membrane fusion, platelet activation, and cell cycle progression.  Specialized forms of PE exist as plasmologens, natural cannabinoid anadimade, and glycosylated PE.  The ECT enzyme acts in the second of three catalysis steps to produce CDP-ethanolamine from phosphoethanolamine (P-Etn).  Study of ECT could help determine the role of plasmologens in disease, specifically lipid disorders such as peroxisomal diseases as well as neuronal degeneration associated with Alzheimer ft.s disease.


Synthetic Peptide: C(157) T K A H H S S Q E M S S E Y R E(172)
Affinity chromatography
Human, Mouse, Non-human primate, Rat
Immunocytochemistry, Immunofluorescence, Western Blot
0.4 mg/mL
tris with 1mg/mL BSA, glycine and 0.02% thimerosal
Q99447, Q922E4, O88637
Ethanolaminephosphate Cytidyltransferase,
100 μg
-20° C, Avoid Freeze/Thaw Cycles
5833, 68671, 89841
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