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eGFP Rabbit anti-Tag, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA118410

Catalog No. 01-670-660


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Description

Description

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

PA1-18410 detects EGFP.PA1-18410 has been successfully used in immunohistochemistry and Western blot procedures.The PA1-18410 immunogen is recombinant EGFP.After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid freeze-thaw cycles.Reconstitute with 100 ul of distilled water. Glycerol (1:1) may be added for additional stability.
Specifications

Specifications

eGFP
Polyclonal
whole serum with no preservative
GFP2; Green Fluoresecent Protein; GFP; Turbo GFP; eGFP; GFP tag
Recombit EGFP.
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
Tag
Immunohistochemistry, Western Blot
Unconjugated
Lyophilized
Rabbit
IgG
RUO
Antibody
Polyclonal
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