eRF1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA528777
PA5-28777 targets eRF1 in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-28777 immunogen is a synthetic peptide corresponding to a region within amino acids 374 and 437 of eRF1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
|Synthetic peptide corresponding to a region within amino acids 374 and 437 of eRF1|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7|
|D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1, eukaryotic peptide chain release factor subunit 1, eukaryotic release factor 1, polypeptide chain release factor 1, protein Cl1, sup45 (yeast omnipotent suppressor 45) homolog-like 1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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