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LMAN1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1074

Catalog No. PA1074

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PA1-074 detects ERGIC-53 in rat, mouse and human samples. PA1-074 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~57.5 kDa protein representing ERGIC-53 in rat brain samples. The PA1-074 immunogen is a synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53. This sequence is conserved in mouse and human. The PA1-074 immunizing peptide (Cat. No. PEP-277) is available for use in neutralization and control experiments.

Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. ERGIC-53 (ER-Golgi intermediate compartment 53) is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. ERGIC-53 is involved in intracellular transport of many glycoproteins. ERGIC-53 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly



Synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53.
Antigen affinity chromatography
Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P49257, Q9D0F3, Q62902
ER-Golgi intermediate compartment 53 kDa protein, Lectin mannose binding-1, p58, Lman1
100 μg
-20° C, Avoid Freeze/Thaw Cycles
116666, 3998, 70361
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