Estrogen Receptor alpha Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1308
PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining. The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. No. PEP-036) is available for use in neutralization and control experiments. Recombit ER alpha expressed in baculovirus system (Cat. No. RP-310) can be purchased for control experiments in Western blot and gel shift assays.Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
|Estrogen Receptor alpha|
|Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.|
|Antigen affinity chromatography|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|ER Alpha, ER, Estradiol receptor, ESR1|
|-20° C, Avoid Freeze/Thaw Cycles|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok