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Estrogen Receptor alpha Rabbit anti-Human, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1308

Catalog No. PA1308


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Description

Description

PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining. The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. No. PEP-036) is available for use in neutralization and control experiments. Recombit ER alpha expressed in baculovirus system (Cat. No. RP-310) can be purchased for control experiments in Western blot and gel shift assays.

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Specifications

Specifications

Estrogen Receptor alpha
Polyclonal
Unconjugated
ESR1
Liquid
ESR1
Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
P03372, P06211
ER Alpha, ER, Estradiol receptor, ESR1
Rabbit
IgG
50 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
2099, 24890
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