FEN1 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA527518
PA5-27518 targets FEN1 in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-27518 immunogen is recombit fragment corresponding to a region within amino acids 169 and 380 of Human FEN1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.The protein encoded by this gene removes 5 ft. overhanging flaps in DNA repair and processes the 5 ft. ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5 ft. end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
|Recombit fragment corresponding to a region within amino acids 169 and 380 of Human FEN1|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7|
|P39748, P39749, Q5XIP6|
|FEN-1, MF1, RAD2, DNase IV, flap endonuclease 1, hFEN-1, maturation factor 1, maturation factor-1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|14156, 2237, 84490|
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