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DYKDDDDK Tag Rabbit anti-Tag, HRP, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1984BHRP

Catalog No. PA1984BHRP


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Description

Description

PA1-983B-HRP detects Flag-tagged proteins in Western Blot applications. Similar epitope as Flag tag from Sigma.

DYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombit DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Specifications

Specifications

DYKDDDDK Tag
Polyclonal
HRP
Liquid
Rabbit
IgG
50 μL
4° C, do not freeze
Primary
Tag
Western Blot
1 mg/mL
PBS with proprietary stabilizer and 0.07% Kathon
DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG Tag
Synthetic peptide corresponding to residues DYKDDDDK
Affinity chromatography
RUO
Antibody
Polyclonal
Documents
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