FLI1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA137705

Catalog No. 01-673-460

Description

This antibody is predicted to react with mouse and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is angiosarcoma, Ewing ft.s sarcoma or placenta tissue.

ETS-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes ETS-1, ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU.1 and PEA3. Members of the ETS gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Specifications

• Antigen: FLI1
• Classification: Polyclonal
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: Q01543, P26323
• Gene Alias: EWSR2, SIC-1, Friend leukemia integration 1 transcription factor, proto-oncogene Fli-1, transcription factor ERGB
• Host Species: Rabbit
• Isotype: IgG
• Quantity: 1 mL
• Storage Requirements: -20° C, Avoid Freeze/Thaw Cycles
• Primary or Secondary: Primary
• Gene ID (Entrez): 14247, 2313

• Applications: Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Gene: FLI1
• Format: Liquid
• Gene Symbols: FLI1
• Immunogen: Synthetic peptide derived from C-terminus of human FLI-1.
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Polyclonal
• Target Species: Human, Mouse