Fluorescein/Oregon Green Goat anti-Chemical, Alexa Fluor® 488, Polyclonal, Invitrogen
Goat Polyclonal Antibody
Manufacturer: Invitrogen A11096
We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes. In addition to their utility in quenching the fluorescence of extracellular fluorescein, the high affinity of Invitrogen ft.s anti-fluorescein antibodies for both fluorescein and the structurally related Oregon GreenTM dye makes them ideal for immunochemical applications. Harmer and Samuel report the fluorescein-anti-fluorescein system provides a sensitive alternative to the biotin-streptavidin methods conventionally used for enzyme-linked immunosorbent assays (ELISAs). Fluorescein has also been found to be an excellent hapten for FISH, yielding sensitive results with extremely low background levels. The Alexa Fluor 488 conjugate can be used to amplify the fluorescence signal while still allowing fluorescein-compatible optics. The approximate absorption (Abs) and fluorescence emission (Em) maxima in nm: 495/519. Our anti-dye antibodies can be used in many different applications. It is a good practice to centrifuge the labeled antibody solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment.This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Fluorescein (FITC) derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility. Fluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications. Fluorescein displays a relatively high rate of photobleaching, a flueorescent signal thaqt is sensitive to pH changes, a relatively broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers. Invitrogen offers a broad range of fluorescein conjugates and derivatives.
|Alexa Fluor™ 488|
|4° C, store in dark|
|0.1M potassium phosphate with 5mM sodium azide; pH 8|
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