FITC Goat anti-Chemical, Polyclonal, Invitrogen
Goat Polyclonal Antibody
Manufacturer: Invitrogen PA126793
PA1-26793 detects Fluorescein. PA1-26793 has been successfully used in ELISA, immunodiffusion, immunohistochemistry (frozen), immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. The PA1-26793 immunogen is Fluorescein, conjugated to goat IgG. Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below, avoiding multiple freeze-thaw cycles.FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|ELISA, Immunodiffusion, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2|
|Fluorescein isothiocyanate; Fluorescein|
|Fluorescein, conjugated to goat IgG.|
|Antigen affinity chromatography|
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