anti-Gαo G-Protein Ab-2, Clone: 2A.3, Epredia™ LabVision

Mouse Monoclonal Antibody

Supplier:  Epredia MS242P

Catalog No. MS242P

Description

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of α-subunits (αo1 and αo2) derived from alternative splicing of a single gene. αo1 differs from αo2 within the C-terminal third of the deduced amino acid sequence.

Host Species: Mouse
Clone: 2A.3
Isotype: IgG1
Cross Reactivity: Human, Cow, Rat, Mouse, and Guinea pig. Others-not known
Epitope: Not determined
Immunogen: Partially purified bovine brain Gαo protein
Molecular Weight: 39-42kDa
Positive Control: IMR-5 cells. Brain
Cellular Localization: Cell membrane

Ab-2 is highly specific to Galphao and shows no cross-reaction with transducin, Gαi1, Gαi2, Gαi3, or Gαs. It reacts equally with the C-terminal splice variants of Gαo (mouse GαoA and GαoB, equivalent to Gαo1 and Gαo2 in other species).

Western Blotting

Specifications

• Antigen: Gαo G-Protein Ab-2
• Concentration: 200μg/mL
• Host Species: Mouse
• Quantity: 1 mL
• Target Species: Bovine, Guinea Pig, Human, Mouse, Rat
• Isotype: IgG1

• Classification: Monoclonal
• Conjugate: Unconjugated
• Immunogen: Partially purified bovine brain Gαo protein
• Primary or Secondary: Primary
• Form: Purified