GAL4 Mouse anti-Yeast, Clone: 5C8-5, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA112905
DescriptionGAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a "zinc finger" sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
|PBS with 0.08% sodium azide|
|Full-length recombit GAL4 expressed in E. coli|
|Immunoprecipitation, Western Blot|
|-20° C, Avoid Freeze/Thaw Cycles|
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