gamma Tubulin Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138817
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.Tubulin gamma, an essential structural protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma- depleted cells fail to form functional spindles and arrest during nuclear division. Tubulin gamma associates with spindle body components Spc97 and Spc98 to form the Tubulin gamma complex. The phosphrylation of Tubulin gamma at Tyr445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1.
|PBS with 1% BSA and 0.1% sodium azide|
|GCP-1, TUBG, TUBGCP1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Synthetic peptide derived from C-terminus of human tubulin gamma protein|
|Antigen affinity chromatography|
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