GLC1D Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137756
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is kidney or testis tissue.Gamma Glutamylcystuene Synthetase (GSH) is the first enzyme and rate limiting step in the slutathione biosynthesis pathway. Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells, GSH is synthesized denovo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.
|PBS with 1% BSA and <0.1% sodium azide|
|Synthetic peptide derived from internal region of heavy subunit of GCS protein|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunohistochemistry (Paraffin), Western Blot|
|GLCLR, GCS light chain, GSC light chain, gamma-ECS regulatory subunit, gamma-glutamylcysteine synthetase regulatory subunit, glutamate--cysteine ligase modifier subunit, glutamate--cysteine ligase regulatory subunit, glutamate-cysteine ligase gamma-glutamylcysteine s|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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