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GFP Rabbit anti-Tag, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1980A

Catalog No. PA1980A


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Description

Description

PA1-980A detects Green Fluorescent Protein (GFP). PA1-980A has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects recombit GFP. PA1-980A immunizing peptide corresponds to the N-terminal amino acid residues 26-39 of Aequorea victoria wild-type GFP. This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. PA1-980A immunizing peptide (Cat. No. PEP-033) is available for use in neutralization and control experiments.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Specifications

Specifications

GFP
Polyclonal
Unconjugated
Liquid
Rabbit
IgG
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
Tag
Immunofluorescence, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
GFP2; Green Fluoresecent Protein; GFP; Turbo GFP; eGFP; GFP tag
Synthetic Peptide: H(26) K F S V S G E G E G D A T(39) C
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Documents
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