GFP Rabbit anti-Tag, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1980A
PA1-980A detects Green Fluorescent Protein (GFP). PA1-980A has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects recombit GFP. PA1-980A immunizing peptide corresponds to the N-terminal amino acid residues 26-39 of Aequorea victoria wild-type GFP. This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. PA1-980A immunizing peptide (Cat. No. PEP-033) is available for use in neutralization and control experiments.The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
|-20° C, Avoid Freeze/Thaw Cycles|
|Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|GFP2; Green Fluoresecent Protein; GFP; Turbo GFP; eGFP; GFP tag|
|Synthetic Peptide: H(26) K F S V S G E G E G D A T(39) C|
|Antigen affinity chromatography|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok