HSPA9 Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137803
This antibody is predicted to react with bovine, mouse, porcine, rabbit and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma.The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.
|PBS with 1% BSA and <0.1% sodium azide; pH 7.4|
|CSA; GRP-75; GRP75; HSPA9B; MOT; MOT2; MTHSP75; PBP74; 75 kDa glucose-regulated protein; heat shock 70kD protein 9B; mortalin; perinuclear; mortalin-2; p66-mortalin; peptide-binding protein 74; stress-70 protein; mitochondrial|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide corresponding to aa 661-679 of human GRP75 protein.|
|Antigen affinity chromatography|
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