GRP94 Rabbit anti-Human, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137807
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.Heat shock protein (HSP) molecular chaperones are environmental stressinducible gene products. The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90beta, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94 (glucose regulated protein 94 kDa), also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.
|PBS with 1% BSA and <0.1% sodium azide; pH 7.4|
|ECGP; GP96; GRP94; TRA1; 94 kDa glucose-regulated protein; GRP-94; Tumor rejection antigen-1; endoplasmin; endothelial cell (HBMEC) glycoprotein; glucose regulated protein; 94 kDa; gp96 homolog; heat shock protein 90 kDa beta member 1; tumor rejection antigen 1|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Immunohistochemistry (Paraffin), Western Blot|
|Synthetic peptide corresponding to C-terminus of human GRP-94 protein|
|Antigen affinity chromatography|
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