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MilliporeSigma™ anti-Glutathione S-Transferase, Polyclonal

Rabbit Polyclonal Antibody

Manufacturer:  MilliporeSigma™ PC53850UL

Catalog No. PC53850UL

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Specifically detects Glutathione S-Transferase Clone: in S. japonicum samples, and it is validated for Affinity Purification, ELISA, Immunoprecipitation, Immuno Blotting

Glutathione S-transferase (GST) is commonly used as an epitope tag in generating fusion proteins. In these systems a~26kDa GST domain isolated fromS. japonicum is cloned in-frame to the gene encoding the protein of interest in the appropriate expression vector system. The resulting fusion protein can then be expressed by eitherE. coli (pGEX expression vector system) or by yeast (pYEX™ expression vector system) in a soluble form. The resulting fusion proteins may then be further purified by immunoprecipitation or affinity chromatography with GST (Ab-1) or directly analyzed by western blotting with the antibody.
Specifications

Specifications

Glutathione S-Transferase
Unconjugated
purified
recombinant glutathione S-transferase from S. japonicum
50μL
2°C to 8°C
Polyclonal
S. japonicum
Affinity Purification, ELISA, Immuno Blotting, Immunoblot, Immunoprecipitation
In PBS, 0.2% Tween™-20 detergent.
Rabbit
IgG
RUO
Primary
Recognizes glutathione S-transferase. Good negative control for rabbit polyclonal antibodies.
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