MilliporeSigma anti-Glutathione S-Transferase, Polyclonal
Rabbit Polyclonal Antibody
Manufacturer: MilliporeSigma PC53850UL
Specifically detects Glutathione S-Transferase Clone: in S. japonicum samples, and it is validated for Affinity Purification, ELISA, Immunoprecipitation, Immuno BlottingGlutathione S-transferase (GST) is commonly used as an epitope tag in generating fusion proteins. In these systems a~26kDa GST domain isolated fromS. japonicum is cloned in-frame to the gene encoding the protein of interest in the appropriate expression vector system. The resulting fusion protein can then be expressed by eitherE. coli (pGEX expression vector system) or by yeast (pYEX expression vector system) in a soluble form. The resulting fusion proteins may then be further purified by immunoprecipitation or affinity chromatography with GST (Ab-1) or directly analyzed by western blotting with the antibody.
|recombinant glutathione S-transferase from S. japonicum|
|2°C to 8°C|
|Affinity Purification, ELISA, Immuno Blotting, Immunoblot, Immunoprecipitation|
|In PBS, 0.2% Tween™-20 detergent.|
|Recognizes glutathione S-transferase. Good negative control for rabbit polyclonal antibodies.|
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