MilliporeSigma anti-GNA13 Clone: 6F6-B5,
Mouse Monoclonal Antibody
Manufacturer: MilliporeSigma ST1629100UG
Specifically detects GNA13 Clone: 6F6-B5 in Human samples, and it is validated for ELISA, Immuno BlottingHeterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an α-, β- and γ-subunit and is bound to GDP in the 'off' state. Ligand binding to a 7-TM receptor causes a conformational change in the receptor, which detaches the G protein. This reaction switches the G protein to an 'on' state, allowing GTP to displace GDP, thus Gα can activate second messenger signaling cascades. There are several types of Gα proteins; Gαs and Gαi bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively. Gαq/11 stimulates PLCβ which catalyzes the hydroylsis of PIP2 to the second messengers IP3 and DAG. Gv12/13 are involved in Rho family GTPase signaling. In addition, some Gβγ subunits have active functions; Gβv coupled to H1 receptors can activate PLA2 and Gβγ coupled to M1 receptors can activate KIR channels. Rapid termination of the cellular response is required once the concentration of the stimulating ligand decreases. GTP bound to Gα is hydrolyzed rapidly to GDP, Gα is converted to its inactive form and re-associates with the β- and γ-subunits returning to the 'off' state.
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|In PBS, pH 7.2.|
|Recognizes the ∽40-45kDa GNA13 protein in HepG2 cells.|
|ELISA, Immuno Blotting, Immunoblot|
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