GOLPH3 Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA520830
A suggested positive control is rat lung tissue lysate. PA5-20830 can be used with blocking peptide PEP-0944.GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.
|A 14 amino acid peptide from near the amino terminus of human GOLPH3.|
|Antigen affinity chromatography|
|Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 0.02% sodium azide|
|Q9H4A6, Q9CRA5, Q9ERE4|
|GOPP1, GPP34, MIDAS; Golgi phosphoprotein 3; coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; golgi protein; golgi-associated protein; mitochondrial DNA absence factor|
|Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|64083, 66629, 78961|
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