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Granzyme B Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA137799

Catalog No. 01-673-531


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Description

Description

This antibody is predicted to react with canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Specifications

Specifications

Granzyme B
Polyclonal
PBS with 1% BSA and 0.1% sodium azide; pH 7.4
P18291, P10144, P04187
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, C11, CTLA-1, T-cell serine protease 1-3E, cathepsin G-like 1, cytotoxic T-lymphocyte proteinase 2, cytotoxic serine protease B, fragmentin 2, fragmentin-2, granzyme B, human lymphocyte protein
Rabbit
IgG
1 mL
-20° C, Avoid Freeze/Thaw Cycles
Primary
3002, 14939, 171528
Immunohistochemistry (Paraffin)
Unconjugated
GZMB
Liquid
GZMB
Synthetic peptide from N-terminus of human granzyme B.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse, Rat
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