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HDAC2 Rabbit anti-Canine, Hamster, Human, Mouse, Non-human primate, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1861

Catalog No. PA1861

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PA1-861 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells. PA1-861 has been successfully used in Western blot, ChIP, immunoprecipitation, immunofluorescence, flow cytometry, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing HDAC2 and a ~40 kDa protein of unknown identity from HeLa cell lysate. Immunocytochemical staining of HDAC2 in HeLa cells with PA1-861 results in nuclear staining. The PA1-861 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. No. PEP-070) is available for use in neutralization and control experiments.

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.


Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2.
Antigen affinity chromatography
Canine, Hamster, Human, Mouse, Non-human primate, Rat
ChIP assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
Q92769, P70288
Histone Deacetylase 2
100 μg
-20° C, Avoid Freeze/Thaw Cycles
100764557, 15182, 3066, 475035, 84577
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