HDAC4 Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1863
PA1-863 detects histone deacetylase 4 (HDAC4) from human cells. PA1-863 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~140 kDa protein representing HDAC4 from HeLa cell lysate. An ~110 kDa protein is also detected with PA1-863 which could correspond to protein degradation product. PA1-863 immunogen is a synthetic peptide corresponding to residues M(1)SSQSHPDGLSGRDQPVE(18) of human HDAC4. PA1-863 can be used with blocking peptide PEP-141 and blocking peptide PEP-095.Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with N-CoR.
|Synthetic peptide corresponding to residues M(1)SSQSHPDGLSGRDQPVE(18) of human HDAC4|
|Antigen affinity chromatography|
|ChIP assay, Immunohistochemistry (Paraffin), Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Histone Deacetylase 4|
|-20° C, Avoid Freeze/Thaw Cycles|
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