Thermo Scientific™ Lab Vision™ Heat Shock Factor 1 (HSF1) Ab-1, Rat Monoclonal Antibody

Ensure accurate, reproducible results in western blotting, immunofluorescence, immunoprecipitation and gel supershift experiments.

Manufacturer:  Thermo Scientific™ RT405P1

Catalog No. RT405P1

Description

Ensure accurate, reproducible results in western blotting, immunofluorescence, immunoprecipitation and gel supershift experiments with Thermo Scientific™ Heat Shock Factor 1 (HSF1) Ab-1, Rat Monoclonal Antibody.

HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents; binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.

Host Species: Rat

Clone: 4B4

Isotype: IgG1

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: aa 425-439 of mouse HSF1

Immunogen: Recombinant mouse HSF1 protein (aa 1-503)

Molecular Weight: 70-85 kDa (depending upon the source and state of cells)

Positive Control: LS174T or MAD109 cells

Cellular Localization: Nuclear

Recommended for:

• Western Blotting
• Immunofluorescence
• Immunoprecipitation (Native only)
• Gel Supershift

Note:

USA: RUO; Int'l: RUO

Specifications

• Antigen: Heat Shock Factor 1 (HSF1) Ab-1
• Classification: Monoclonal
• Concentration: 200µg/mL
• Format: Concentrated
• Immunogen: Recombinant mouse HSF1 protein (aa1-503)
• Quantity: 500μL
• Research Discipline: Stress Response
• Primary or Secondary: Primary
• Target Species: Human, Murine

• Applications: Immunoprecipitation, Western Blot
• Clone: 4B4
• Conjugate: Unconjugated
• Host Species: Rat
• Isotype: IgG1
• Regulatory Status: RUO
• Type: Primary
• Monoclonal or Polyclonal: Monoclonal