HSP27 Rabbit anti-Human, Non-human primate, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1017
PA1-017 detects heat shock protein 27(hsp27) from rat, mouse, monkey, and human tissue tissues. PA1-017 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects a ~27 kDa protein which corresponds to hsp27 from HeLa cells. Immunocytochemical staining of hsp27 in HeLa cells with this antibody results in diffuse cytoplasmic staining before heat shock and diffuse cytoplasmic along with punctate staining patterns after. The PA1-017 immunogen is a synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27. This peptide is 81% conserved in mice, rats, and hamsters. The phosphorylated peptide (Cat. No. PEP-187) and non-phosphorylated peptide (Cat. No. PEP-240) are available for use in neutralization and control experiments.Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP27 also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP ft.s and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that HSP27 may be a useful marker in classifying various hormone sensitive tumors.
|Synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27.|
|Antigen affinity chromatography|
|Human, Non-human primate, Rat|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Heat shock protein beta-1; HspB1; HSP25; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein; HSPB1; HSP27|
|-20° C, Avoid Freeze/Thaw Cycles|
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