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HIF1A Mouse anti-Bovine, Canine, Human, Mouse, Rat, Clone: ESEE122, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen MA116518

Catalog No. AMPA6518


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Description

Description

Suggested positive control: Cos7 CoCl2-treated nuclear extract.

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
Specifications

Specifications

HIF1A
Monoclonal
1 mg/mL
tris glycine with 150mM NaCl and 0.05% sodium azide
Q9XTA5, Q16665, Q61221, O35800
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
Mouse
IgG1
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
15251, 281814, 29560, 3091, 480348
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
ESEE122
Unconjugated
HIF1A
Liquid
HIF1A
Residues 329-530 of human HIF-1 alpha.
Protein G
RUO
Antibody
Monoclonal
Bovine, Canine, Human, Mouse, Rat
SDS
Documents
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