Histone H3.3 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA527507
PA5-27507 targets Histone H3.3 in IF and WB applications and shows reactivity with Human and mouse samples. The PA5-27507 immunogen is recombit protein fragment corresponding to a region within amino acids 1 and 105 of Human Histone H3.3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
|Recombit protein fragment corresponding to a region within amino acids 1 and 105 of Human Histone H3.3|
|Antigen affinity chromatography|
|ChIP assay, Immunocytochemistry, Immunofluorescence, Western Blot|
|0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7|
|Histone H3, H3.3A, H3.3B, H3F3, H3F3A, H3F3B|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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