HIV1 Tat Mouse anti-Virus, Clone: N8, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA171513
This antibody reacts with HIV-1 Tat HXB2, BH10, IIIB and HAN strains. It has been mapped to amino acids 59-67 (PQDSQTHQV – B subtype a.a. consensus). MA1-71513 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat. This clone may also be referred to as N8 (Tat N8).HIV-1 Tat ("Trans-Activator of Transcription") consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.
|PBS with 0.1% sodium azide; pH 7.4|
|Human immunodeficiency virus; HIV-1 Tat|
|Recombit Tat protein of HIV-1 HAN purified from E. coli|
|ChIP assay, ELISA, Western Blot|
|-20° C, Avoid Freeze/Thaw Cycles|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok