hPrp16 Mouse anti-Human, Unlabeled, Clone: 50, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 611323
Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first step involves cleavage of the 5 ft. exon and the production of a lariat intermediate. In the second step, the 3 ft.-splice site is cleaved and the exons are fused with concomitant release of the intron lariat. The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors. Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step. Human
homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), and Prp18p (hPrp18). Prp16 is a DExD/DExH-box family RNA dependent ATPase, which can unwind RNA duplexes. hPrp16 is 41% identical to its yeast counterpart and chimeric yeast-human Prp16 can rescue a yeast Prp16 knockout strain. The non-conserved N-terminal region of hPrp16 is essential for viability, required for nuclear localization, and capable of binding to the splicesome during the second catalytic step. Thus, hPrp16 is a non-snRNP that is critical for spliceosomal function in the process of pre-mRNA splicing.
Host Species: Mouse
Species Reactivity: Human
Immunogen: Human hPrp16 1002-1203
Formula Weight [Chemical]: 140kDa
Immunofluorescence, Western Blotting
|Immunofluorescence, Western Blot|
|Human hPrp16 1002-1203|
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