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HSF1 Rabbit anti-C. elegans, Human, Mouse, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA3017

Catalog No. PIPA3017


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Description

Description

PA3-017 detects heat shock factor 1 (HSF-1) from human, mouse and rat tissues and cells. PA3-017 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, ELISA, and gel super shift protocols. By Western blot, this antibody detects an ~83 kDa protein representing HSF-1 from NIH-3T3 cell lysate. The PA3-017 immunogen is recombit human HSF-1 protein expressed in E. coli.

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
Specifications

Specifications

HSF1
Polyclonal
whole serum diluted in PBS with 0.05% sodium azide
Q00613, P38532
HSF 1; HSTF 1; heat shock factor protein 1
Rabbit
IgG
RUO
Antibody
Polyclonal
C. elegans, Human, Mouse, Rat
ELISA, Gel Shift, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Unconjugated
HSF1
Liquid
HSF1
Recombit human HSF1 expressed in E. coli.
100 μL
-20° C, Avoid Freeze/Thaw Cycles
Primary
15499, 3297, 79245
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