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HuC/HuD Mouse anti-Avian, Chicken, Human, Zebrafish, Clone: 16A11, Invitrogen™ Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen A21271

Catalog No. A21271

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This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle. Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles. For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. A-21271 was successfully used to detect HuC/HuD in neurons differentiated from H9 ESC derived NSCs.

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

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ELAVL3, elavl4
Human HuC/HuD neuronal protein.
Avian, Chicken, Human, Zebrafish
Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
Antibody with no preservative
P26378, Q14576
ELAV-like protein 3, Hu-antigen C, HuC, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, ELAVL3, HUC, PLE21, ELAV-like protein 4, Hu-antigen D, HuD, Paraneoplastic encephalomyelitis antigen HuD, ELAVL4, HUD, PNEM
IgG2b, kappa
100 μg
1995, 1996, 30732, 30737, 395634
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