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CD4 Mouse anti-Human, Brilliant Violet 605, Clone: RPA-T4, BD
Mouse Monoclonal Antibody
Supplier: BD Biosciences 562658
Description
The RPA-T4 monoclonal antibody specifically binds to CD4, a 59 kDa single-chain transmembrane glycoprotein that is expressed on T-helper/inducer cell populations. CD4 is also expressed on thymocytes subsets and at lower levels on monocytes and macrophages. CD4 functions as a co-receptor in MHC class II-restricted antigen-induced T cell activation and as a receptor for human immunodeficiency viruses (HIV). This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
Specifications
CD4 | |
Monoclonal | |
Brilliant Violet 605 | |
Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
Affinity Purified | |
RUO | |
Human | |
IgG1 κ |
Flow Cytometry | |
RPA-T4 | |
L3T4; T-cell surface antigen T4/Leu-3; W3/25; CD4 antigen (p55) | |
Mouse | |
100 Tests | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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