anti- IgG2a, κ, Clone: R35-95, Rat, APC-R700, Isotype Control, BD
Manufacturer: BD Biosciences 564982
The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.
This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45nm filter).
Flow Cytometry, Isotype Control
|Rat IgG2a, kappa|
|Rat (LOU) IgG2a, κ|
|4°C, protect from prolonged exposure to light, DO NOT FREEZE|
|Mouse Pooled Immunoglobulin|
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