anti- IgG2a, κ, Clone: R35-95, Rat, APC-R700, Isotype Control, BD
Manufacturer: BD Biosciences 564982
The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.
This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor (eg, 730/45nm filter).
Flow Cytometry, Isotype Control
|Rat IgG2a, kappa|
|Rat (LOU) IgG2a, κ|
|4°C, protect from prolonged exposure to light, DO NOT FREEZE|
|Mouse Pooled Immunoglobulin|
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