anti-IgG2a, κ, V500, Clone: R35-95, BD

Supplier:  BD Biosciences 560786

Catalog No. BDB560786

Description

The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues. The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500nm. BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD Horizon V500, visit bdbiosciences.com/colors. When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls. Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Flow Cytometry, Isotype Control

Specifications

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Host Species: Rat
• Quantity: 0.1 mg
• Content And Storage: Undiluted at 4°C. Protect from exposure to light. Do not freeze.

• Clone: R35-95
• Conjugate: Violet Fluor 500 (V500)
• Purification Method: Affinity Purified
• Target Species: Human, Mouse
• Isotype: IgG2a κ