IkB alpha (Cleaved) Mouse anti-Human, Clone: 5D1623, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA141251
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.Suggested positive control: anti-Fas treated Jurkat (30 min).
|IkB alpha (Cleaved)|
|PBS with 0.05% BSA and 0.05% sodium azide|
|IKBA, MAD-3, NFKBI|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Caspase-3 mediated cleavage site of IkBa.|
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