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anti-IL-1 beta/IL-1F2, HRP, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP142769

Catalog No. NBP142769


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Description

Description

IL-1 beta/IL-1F2 Polyclonal antibody specifically detects IL-1 beta/IL-1F2 in Mouse, Rat samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.
Specifications

Specifications

IL-1 beta/IL-1F2
LYOPH
Western Blot 1:1000-1:5000, ELISA 1:10000-1:50000, Immunohistochemistry 1:500-1:2500, Immunohistochemistry-Paraffin 1:10-1:500
Purified
IL1B
This antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta/IL-1F2 produced in E.coli. The MW of recombinant mouse IL-1 beta was 17 kDa.
17 kDa
0.1 mg
Adaptive Immunity, Apoptosis, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Primary
3553
Reconstitute with 100 ul deionized water.
ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
HRP
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Gentamicin Sulfate
catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Rabbit
IgG
Ion exchange chromatography
RUO
Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Polyclonal
This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
Mouse, Rat
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