IL-1 beta/IL-1F2 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NB600633

Catalog No. NB600633

Description

IL-1 beta/IL-1F2 Polyclonal antibody specifically detects IL-1 beta/IL-1F2 in Human, Mouse, Rat, Porcine, Canine, Monkey, Golden Syrian Hamster samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoassay, Immunohistochemistry Whole-Mount.

Specifications

• Antigen: IL-1 beta/IL-1F2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with No Preservative
• Gene Alias: catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
• Host Species: Rabbit
• Molecular Weight of Antigen: 30.75 kDa
• Quantity: 0.1 mg
• Research Discipline: Adaptive Immunity, Apoptosis, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity, Metastasis
• Gene ID (Entrez): 3553
• Target Species: Human, Mouse, Rat, Porcine, Canine, Monkey, Golden Syrian Hamster
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Dot Blot, Immunoassay, Immunohistochemistry
• Concentration: 2 mg/ml
• Dilution: Western Blot 1:1000, Flow Cytometry, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen, Dot Blot, Electron Microscopy 1:10-1:500, Immunohistochemistry Whole-Mount
• Gene Accession No.: P01584
• Gene Symbols: IL1B
• Immunogen: This IL-1 beta/IL-1F2 Antibody was prepared by repeated immunizations with recombinant human IL-1 beta/IL-1F2 produced in E.coli. The MW of the recombinant 153 aa IL-1 beta/IL-1F2 was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta/IL-1F2 converting enzyme (ICE, capase-1). (Uniprot: P01584)
• Purification Method: Whole Antiserum
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody is primarily directed against mature, 17,000 MW human IL-1 beta/IL-1F2and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1 beta/IL-1F2in the same formats using similar dilutions. The antiserum does not recognize human IL-1alpha. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta/IL-1F2containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1beta precursor molecule. Denatured 31,000 precursor IL-1 beta/IL-1F2will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta/IL-1F2exposing epitopes not exposed in the nat
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only